Table 2.

Method validation data for canine plasma LC-MS/MS assay.

	CBD	6-OH-CBD	7-OH-CBD	THC	11-OH-THC	THC-COOH	CBC
Internal standard	CBD-D3	7-OH-CBD-D9	7-OH-CBD-D9	THC-D3	11-OH-THC-D3	THC-COOH-D3	CBC-D9
Standard curve (ng/mL)	1.97–250	1.97–250	3.91–250	1.97–250	1.97–250	1.97–250	1.97–250
QC range (ng/mL)	5–175	5–175	10–175	5–175	5–175	5–175	5–175
LLOQ (ng/mL)	1.97	1.97	3.91	1.97	1.97	1.97	1.97
LOD (ng/mL)	0.98	0.98	0.98	0.98	0.98	0.98	0.98
Average cannabinoidrecovery (%)	91.15	79.43	87.11	95.24	92.51	93.60	86.62
Intraday accuracy (%)	LQC = 113.4 MQC = 103.5 HQC = 103.8	LQC = 107.7 MQC = 106.9 HQC = 103.5	LQC = 107.5 MQC = 103.2 HQC = 99.9	LQC = 113.5 MQC = 103.7 HQC = 105.6	LQC = 109.8 MQC = 106.8 HQC = 105.4	LQC = 112.9 MQC = 105.2 HQC = 104.2	LQC = 113.5 MQC = 105.7 HQC = 106.6
Intraday precision (%)	LQC = 0.32 MQC = 3.8 HQC = 2.1	LQC = 4.7 MQC = 3.6 HQC = 1.7	LQC = 3.2 MQC = 1.7 HQC = 1.4	LQC = 2.1 MQC = 2.4 HQC = 1.0	LQC = 5.5 MQC = 3.3 HQC = 2.6	LQC = 1.1 MQC = 3.8 HQC = 1.5	LQC = 1.2 MQC = 4.4 HQC = 2.5
Interday accuracy (%)	LQC = 113.8 MQC = 103.9 HQC = 104.0	LQC = 108.1 MQC = 104.2 HQC = 100.4	LQC = 104.5 MQC = 102.6 HQC = 99.0	LQC = 111.4 MQC = 102.3 HQC = 103.1	LQC = 110.0 MQC = 104.0 HQC = 104.3	LQC = 113.7 MQC = 103.6 HQC = 103.1	LQC = 113.1 MQC = 103.9 HQC = 104.1
Interday precision (%)	LQC = 0.56 MQC = 4.5 HQC = 2.7	LQC = 2.9 MQC = 2.7 HQC = 2.6	LQC = 4.6 MQC = 2.2 HQC = 2.4	LQC = 2.7 MQC = 2.9 HQC = 1.8	LQC = 4.5 MQC = 4.2 HQC = 2.7	LQC = 1.9 MQC = 3.1 HQC = 2.2	LQC = 1.1 MQC = 2.9 HQC = 1.7

LQC, low-quality control (5 ng/mL for CBD, CBC, 11-OH-THC, THC-COOH, and 6-OH-CBD; 10 ng/mL for THC and 7-OH-CBD); MQC, medium-quality control (100 ng/mL); HQC, high-quality control (175 ng/mL).