FIGURE 1.

SARS-CoV-2 nsp1 sequence analysis. SARS-CoV-2 genomic sequences of high quality (47,427 in total) were collected from the GISAID database. Sequence alignment was performed using the EMBOSS diffseq program with the strain Wuhan-Hu-1 as the reference. According to the alignment, the nsp1 region was found to be highly conservative. Most of the nsp1 sequences are identical to that of the Wuhan-Hu-1 strain without any mutation. Only a few nsp1 protein sequences (1,129 of the analyzed 47,427 sequences) have one or two point mutations, compared to the reference strain. The detailed numbers and ratios of the nsp1 protein sequences with or without mutation were displayed in panel (A). The identified mutation sites and the numbers (≥3) of the sequences harboring the indicated point mutation were summarized in panel (B). Note that most of the mutations were found in only one or two sequences and are not shown in panel (B). See also Supplementary Table S1 and the descriptions in the text.