TABLE 1.
Physicochemical properties of SARS-CoV-2 and SARS-CoV nsp1.
| Parametera | SARS-CoV-2 nsp1 | SARS-CoV nsp1 |
| Number of aa | 180 | 180 |
| Molecular weight Theoretical pI |
19775.31 5.36 |
19640.12 5.35 |
| Formula | C872H1383N247O270S4 | C862H1382N246O272S3 |
| No. of negatively charged residues | 27 (Asp + Glu) | 26 (Asp + Glu) |
| No. of positively charged residues | 19 (Arg + Lys) | 18 (Arg + Lys) |
| Estimated half-lifeb | 30 h | 30 h |
| Instability index | 28.83 (classified as stable) | 27.31 (classified as stable) |
| Aliphatic index | 89.72 | 97.89 |
| Grand average of hydropathicity | −0.378 | −0.357 |
aThe physicochemical parameter values of nsp1 were deduced by the ProtParam tool using the strains SARS-CoV-2 Wuhan-hu-1 and SARS-CoV BJ01 as the references, respectively. bPredicted in the context of mammalian reticulocyte model in vitro.