Glucose, pyruvate, and lactate concentrations in different media used for ovarian tissue transportation. Ovarian fragments of 1 × 1.5 × 0.5 cm were stored at 4 °C in IVF medium, L-15, or NaCl for the indicated times, at which glucose, pyruvate, and lactate concentrations were determined in incubation media using a CMA600 enzymatic analyzer (n = 6). Asterisk indicates p < 0.05 for a given metabolite between 0 and 24 h