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pH and glucose, lactate, and pyruvate concentrations after ovarian tissue transportation in different media. Ovarian fragments of 1 × 1.5 × 0.5 cm were stored at 4 °C in 20 mL of IVF medium, L-15, or PBS for the indicated times, at which pH values were measured using a pH meter, and glucose, pyruvate, and lactate concentrations were determined in incubation media using a CMA600 enzymatic analyzer (n = 4). Asterisk indicates p < 0.05 for a given metabolite between 0, 1, and 24 h
