Cell viability assessed by flow cytometry after ovarian tissue transportation in different media. Ovarian fragments of 1 × 1.5 × 0.5 cm were stored at 4 °C in 20 mL of IVF medium, L-15, or PBS for the indicated times. A piece of tissue was dissociated and cell viability was assessed with annexin V/propidium iodide with flow cytometry. Live cells are shown in green, apoptotic cells in red, necrotic cells in blue, and debris in gray