Fig. 2.

Schematic design for identification of important miRNAs for follicle development. miRNA in follicular fluid derived from small antral follicles (SFFs) and large antral follicles (LFF) were determined by small RNA-seq. Overlapped 250 miRNAs between SFF and LFF were filtered down to 26 miRNA groups by comparison with the upstream regulator miRNA (42 miRNA groups) for follicle development. The upstream regulators were predicted from differentially expressed genes (1751 genes; DDJB, DRA004449) in granulosa cells between small and large follicles. The 26 miRNAs were also filtered down to four miRNAs by comparison with upstream regulator miRNAs (14 miRNA groups) for follicle qualities. The upstream regulators were predicted from differentially expressed genes (174 genes; DDJB, DRA006323) in granulosa cells between good and poor follicles. A detailed explanation (left) and the corresponding Venn diagram (right) are provided