Table 2.

Oocytes grown with FFs, exosome-free FF, control mimic, or miRNA-mimics

Groups	Antral formation (%)	Oocyte diameter (μm)**	Rate of SN (%)	H4K12 acetylation	Rate of blastulation (%)
Control*	90.0 ± 3.7	123.4 ± 0.6a	82.2 ± 5.1	1.0 ± 0.1a	5.6 ± 2.6a
Small-FF	90.0 ± 2.6	132.3 ± 0.7b	90.5 ± 1.9	2.5 ± 0.2b	23.6 ± 3.6b
Small-FF	75.6 ± 0.1	126.6 ± 0.7a	92.5 ± 2.5a	1.0 ± 0.1a	23.6 ± 3.6
Exo-free FF	84.4 ± 0.1	116.9 ± 1.0b	59.3 ± 6.9b	0.7 ± 0.0b	15.5 ± 4.6
Control	91.2 ± 2.2	123.7 ± 1.4	69.4 ± 2.0a	1.0 ± 0.1	6.9 ± 2.8a
miR-17 mimic	95.8 ± 1.6	124.1 ± 1.1	85.8 ± 2.9b	1.2 ± 0.1	14.7 ± 1.8b
Control	88.9 ± 4.1	123.5 ± 1.1	79.6 ± 6.9	1.0 ± 0.1	9.1 ± 2.4
miR-27b mimic	88.9 ± 2.8	125.6 ± 0.9	78.8 ± 7.7	1.2 ± 0.1	20.4 ± 6.5
Control	86.1 ± 1.9a	121.6 ± 1.7a	72.0 ± 6.1a	1.0 ± 0.1a	9.1 ± 2.4a
miR-92a mimic	93.2 ± 1.9b	129.0 ± 0.9b	85.9 ± 2.9b	1.3 ± 0.1b	22.0 ± 5.5b
Control	91.7 ± 2.2	123.7 ± 1.4	69.4 ± 2.0a	1.0 ± 0.1	6.9 ± 2.8
miR-145 mimic	91.7 ± 2.2	125.8 ± 0.8	81.7 ± 0.7b	0.9 ± 0.1	12.3 ± 3.9
Small-FF	×	○	×	○	○
Exo-free FF	×	○	○	○	×
miR-17 mimic	×	×	○	×	○
miR-27b mimic	×	×	×	×	×
miR-92a mimic	○	○	○	○	○
miR-145 mimic	×	×	○	×	×

Small-FF, small follicular fluids; Exo-free FF, exosome-depleted follicular fluids. Data are presented means ± standard error of the mean (SEM). OGCs were cultured in IVG medium containing 3 mg/ml BSA (control*) or 10% small-FF, 10% small-FF or 10% exosome depleted FF, negative control mimic (control) or miRNA mimics. **, oocyte diameter is excluding zona pellucida; a–b, P < 0.05; ○, means significant difference; ×, means comparable with control