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. 2020 Sep 29;10:553045. doi: 10.3389/fonc.2020.553045

Figure 6.

Figure 6

HNRNPA2B1 promotes ESCC cell proliferation. (A) The HNRNPA2B1 knockdown efficiency was verified at the mRNA levels in ECA109 and TE10 cells by qRT-PCR assay. (B) The HNRNPA2B1 knockdown efficiency was verified at the protein levels in ECA109 and TE10 cells by Western blot assay. (C) CCK8 assays showed the growth of ECA109 and TE10 cells upon HNRNPA2B1 knockdown. (D) Knockdown of HNRNPA2B1 impaired the colony formation ability of ECA109 and TE10 cells (upper panel). Quantification of the colony formation assay results (bottom panel). (E) EdU assays showed the growth of ECA109 and TE10 cells upon HNRNPA2B1 knockdown (left panel). Quantification of the EdU-positive cell results (right panel). Scale bars, 100 μm. *P < 0.05, **P < 0.01, and ***P < 0.001.