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The entire proteomics experimental process. Hippocampus samples were reacted with different isotope-labeling TMT regents after immunoaffinity depletion of high-abundance plasma proteins, SDS-PAGE separation, and FASP digestion. Samples were mixed and peptides fractured by high pH reverse-phase chromatography. Finally, LC–MS/MS was used for high-throughput screening of samples. Peptides were then analyzed for function using multiple bioinformatics tools.
