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. 2020 Oct 12;6:37. doi: 10.1038/s41522-020-00148-6

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — Role of different polysaccharides in competition for biofilm formation. a Representative 24 h 3D images of mixed biofilms including the dsRed-tagged PssUMAF0158 wild-type and GFP-tagged matrix mutants. The obtained images were analysed with the Leica Application Suite (Mannheim, Germany) and the IMARIS software package (Bitplane, Switzerland). Scale bar 20 µm. b Percentage of the area occupied by the wild-type and the respective mutants after 24 h of competition calculated with IMARIS software. c Percentage of the volume occupied by the wild-type and the respective mutants after 24 h of competition calculated with IMARIS software. The dsRed-tagged PssUMAF0158 wild-type (PssUMAF0158 wt), GFP-tagged PssUMAF0158 cellulose mutant (ΔwssE), GFP-tagged PssUMAF0158 Psl-like polysaccharide mutant (ΔpslE) and GFP-tagged PssUMAF0158 ΔwssE,pslE double mutant (ΔwssE,pslE) were tested. A minimal of two replicates and three independent experiments were performed. Statistical significance was assessed by two-tailed Mann–Whitney test (*p < 0.05, **p < 0.01, ***p < 0.001). Error bars show the standard error of the mean (s.e.m.).