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. 2020 Oct 12;6:37. doi: 10.1038/s41522-020-00148-6

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 6 — Effect of polysaccharide production on swarming motility. a Representative images of swarming plates incubated at 25 °C at 48 h postinoculation. b Swarm motility area after 48 h of growth at 25 °C. The PssUMAF0158 wild-type (PssUMAF0158 wt), PssUMAF0158 alginate mutant (Δalg8), PssUMAF0158 cellulose mutant (ΔwssE), PssUMAF0158 Psl-like polysaccharide mutant (ΔpslE), Psl-like complemented strain (ΔpslE + pBBRpslE), pslE overexpression strain (wt + pBBRpslE) and vector control (wt + pBBR1MCS5) were tested. Three plates per experiment, and three independent experiments were performed. Statistical analysis was performed using ANOVA with the Bonferroni correction test. Three replicates, and three independent experiments were performed. Different letters represent statistically significant differences, p < 0.05. Error bars show the standard error of the mean (s.e.m.).