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. 2020 Oct 12;10:17048. doi: 10.1038/s41598-020-73863-x

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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(A) 3D confocal laser scanning images of a 3D printed hard scaffold (TCP/HA 80:20) to be used for the Bone Portion of the Plug. (B)The proliferation rate of human osteoblasts on the scaffolds, as well as the standard cell culture plate (control group). N0 initial number of the cells, N number of cells at each time point. Statistical significance *: p < 0.05, **: p < 0.01, p-values in Day 3: 0.006, Day 7: 0.011, Day 14: 0.006 and Day 21: 0.023. (C–F) The fluorescence images of the attached osteoblasts on the scaffolds after (C) 3, (D) 7, (E) 14 and (F) 21 days.