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. 2020 Oct 12;10:16976. doi: 10.1038/s41598-020-73981-6

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Comparative efficiency of hybridization methods. (A) Comparison of LFD results by different hybridization methods. In Method I (post-LAMP), LAMP products from probe-free reactions of 1 pg, 100 fg, 10 fg and 1 fg Mtb-DNA templates were hybridized with the conventional probe for 5 min followed by LFD the assay. In Method II (in-LAMP), LAMP reactions containing the modified probe were carried out to completion and immediately subject to LFD assay. Both hybridization methods shared identical sensitivity. N: negative control (DNase-free water). (B) Corresponding AGE results for the LAMP amplicons as shown in (A).