Table 2.

Demography and Mtb status of samples as determined by the reference assay (standard culture), and the SH-LAMP-LFD protocol.

Source	N	Sex (M/F)	Average age (M/F)	Previous TB-Treatment (Unk/no/yes)	Anti-HIV (Unk/N/P)	Origin (North/Central/ North-east/South)	Mtb status
							Positive by reference/ SH-LAMP-LFD	Negative by reference/ SH-LAMP-LFD
Chulalongkorn hospital	28	N/A	N/A	N/A	N/A	N/A	28/28	0/0
TB Laboratory, BIOTECa	16	9/7	36.5/42	6/6/4	9/6/1	0/10/4/2	16/16	0/0
Siriraj hospital	30	18/12	40/44	9/15/6	17/13/0	0/19/9/2	30/30	0/0
Faculty of tropical medicine, Mahidol Universityb	30	18/12	72.2/54.8	N/A	N/A	N/A	0/0	30/30

^aNational Center for Genetic Engineering and Biotechnology.

^bThe total number of samples from this institute is 30 which are derived from 9 Influenza-infected patients: 7 for Type H3N2, and 2 for Type H1N1. The samples were prepared in various replicates in a time-and-place-independent manner prior to being given blind for the purpose of reliability testing.

N, M, F, Unk, N, P, N/A: total numbers of samples, male, female, unknown, negative, positive, not available, respectively.

These data were used to evaluate the diagnostic performance of the SH-LAMP-LFD assay (Table 3). All samples were sourced from Thailand.