Fig. 10. Topical treatment with NOTCH1 inhibitors impairs cancer/stromal cell expansion in vivo.

a DsRed2-expressing SCC13 cells admixed with CAFs (CAF13) were injected into ears of NOD/SCID mice, followed by topical treatment with DBZ (1 mM) or Ethanol vehicle alone daily for 1 week starting 24 h after injection. Mice were killed 15 days after injection. Shown are images of two ear pairs 15 days after injection, quantification of red fluorescence signal (normalized to day 1 after injection) corresponding to SCC13 cell expansion and tumor volumes (V = (length × width²) × 0.5). Statistical analysis of fluorescence intensity values over time course of the experiment was calculated by Two-way ANOVA, *p < 0.05. Tumor volume values are indicated as dots with mean ± s.d. two-tailed unpaired t-test, *p < 0.05. n(CTR ear lesion) = 3, n(DBZ ear lesion) = 3. b–d Representative images and quantification of immunofluorescence analysis of ear lesions with antibodies against the indicated proteins as in Fig. 9b–d. Scale bar, 50 μm. Values for each lesion are indicated as dots with mean ± s.d. two-tailed unpaired t-test, **p < 0.01, ***p < 0.001. n(CTR ear lesion) = 3, n(DBZ ear lesion) = 3.