FIGURE 2.

Pyrosequencing of 14 CpG sites across the INSR promoter in gluteal (GSAT) and abdominal (ASAT) adipose tissue. (A) Schematic diagram of the INSR gene illustrating CpG sites in the promoter region that were analyzed. The distance between the CpG sites and the transcription start site is indicated. CpG specific methylation levels in GSAT (B) and ASAT (C) of normal-weight and obese black and white women. Cumulative DNA methylation levels across all 14 CpG sites in GSAT (D) and ASAT (E). Data are presented as the mean and SEM (n = 8–11 per group).