FIGURE 3.

Knockdown of p53 in TP53 non-mutant pancreatic cancer cells promoted cancer progression in vitro and in vivo. KP4 and PANC-0327 cells were infected with control or p53-knockdown lentiviruses. (A) p53 expression was analyzed by western blotting. (B) The cell proliferation assay was performed at the indicated time points. (C) Representative micrographs of cell migration assays at 48 h (left) and quantification results (right). (D) Representative micrographs of cell invasion assays (left) and quantification results (right). Data in panels (B–D) are shown as the mean ± SEM of 3 independent experiments. (E) Representative images, volumes and weights of subcutaneous xenografts of KP4 cells with p53 knockdown or control. Data represent means ± SEM for 5 mice per group. **P < 0.01.