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. 2020 Sep 29;10:594224. doi: 10.3389/fonc.2020.594224

FIGURE 4.

FIGURE 4

p53 expression is associated with snoRNA-mediated ribosome maturation. (A) Kaplan–Meier survival curves for PDAC patients according to the snoRNA family genes in tumor tissues, and significance was calculated using the log-rank test. (B,D) Overexpression of p53 in TP53-mutant cells in vitro (B) and in vivo (D) promotes rRNA processing by detecting 5’ETS-18S and ITS–28S using quantitative reverse transcriptase PCR assays in PANC-1 (left) and Patu-8988 cells (right). (C,E) Knockdown p53 in TP53 non-mutant cells in vitro (C) and in vivo (E) suppresses rRNA processing by detecting 5’ETS-18S and ITS–28S using quantitative reverse transcriptase PCR assays in KP4 (left) and PANC-0327 cells (right). The data are shown as the mean ± SEM of 3 independent experiments. **P < 0.01.