FIGURE 5.

STRAP knockdown suppressed progression of TP53-mutant Patu-8988 cells by inhibiting snoRNA-mediated rRNA maturation. Patu-8988 cells with p53 overexpression or control vector were infected with control or STRAP-knockdown lentiviruses. (A) p53 and STRAP expression were analyzed by western blotting. (B,G) rRNA processing in vitro (B) and in vivo (G) by detecting 5’ETS-18S and ITS–28S using quantitative reverse transcriptase PCR assays. The data are shown as the mean ± SEM of 3 independent experiments. (C) The cell proliferation assay was performed at the indicated time points. (D) Representative micrographs of cell migration assays at 48 h (top) and quantification results (bottom). (E) Representative micrographs of cell invasion assays (left) and quantification results (right). Data in panels (C–E) are shown as the mean ± SEM of 3 independent experiments. (F) Representative volumes of subcutaneous xenografts of Patu-8988 cells with overexpressing p53 or control injected intratumorally with control or STRAP- knockdown lentivirus. Data represent means ± SEM for 5 mice per group. **P < 0.01.