FIGURE 2:

WAVE1 and WAVE2 have distinct effects on F-actin density at the leading edge. (A) Representative cell images showing distribution of Arp2/3 complex (p16/ARPC5 subunit) at the leading edge determined by immunofluorescence. Scale bar, 10 µm. Highlighted regions (dotted boxes) shown at higher magnification. Scale bar, 3 µm. (B) Density of Arp2/3 complex (mean ± SD) in lamellipodia, quantified from cell images as in A. Data pooled from three independent experiments (left to right: n = 162, 159, 151, 165, 146, 156 cells). (C) Representative cell images showing F-actin stained with Alexa⁵⁶⁸-phalloidin. Scale bar, 10 µm. Highlighted regions (dotted boxes) shown at higher magnification. Scale bar, 3 µm. (D) Density of F-actin at the leading edge, quantified from cell images as in C. Mean ± SD shown. Data pooled from three independent experiments (left to right: n = 168, 175, 161, 139, 157, 193 cells). One-way ANOVA and Tukey’s multiple comparison tests were performed in B and D. *, p < 0.05; **, p < 0.01; ****, p < 0.0001; ns (p > 0.05), not significant.