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Protrusion dynamics at the leading edge. Representative phase contrast time-lapse imaging for 5 min of WT, WAVE1 KO, WAVE2 KO, and WAVE1/2 KO B16-F1 cells, shown at 25 frames per second. Zoom shows highlighted region in right most panel at higher magnification (yellow box), with green arrowhead tracking one of the extending filopodia-like structures, while the yellow arrowhead tracking one of the retracting structures. Scale bars, 10 μm.
