FIGURE 4:

Delocalization of ciliary lipidated membrane proteins in the absence of MKS1 or B9D2. (A–J) Control RPE1 cells (A, F), the MKS1-KO cell lines #MKS1-2-2 (B, G) and #MKS1-3-13 (C, H), and the B9D2-KO cell lines #B9D2-1-5 (D, I) and #B9D2-3-6 (E, J) were serum starved for 24 h to induce ciliogenesis and triple immunostained with an antibody against either ARL13B (A–E) or INPP5E (F–J) and against either Ac-tubulin (A′–E′) or GT335 (F′–J′), which recognizes polyglutamylated tubulin, and against either γ-tubulin (A′′–E′′) or FOP (F′′–J′′). Scale bars, 5 µm. (K, L) The relative ciliary staining intensities of ARL13B and INPP5E in control, MKS1-KO, and B9D2-KO cells were estimated and expressed as scatter plots. Different colored dots represent three independent experiments, horizontal lines are means, and error bars are SD. Statistical significances among multiple cell lines were calculated using one-way ANOVA followed by the Dunnett multiple comparison test.