FIGURE 7:

Interdependent TZ localization of the B9D proteins. (A–I) Control RPE1 cells (A, D, G), MKS1-KO cells (#MKS1-3-13) (B, E, H), or B9D2-KO cells (#B9D2-3-6) (C, F, I) stably expressing EGFP-fused MKS1 (A–C), B9D1 (D–F), or B9D2 (G–I) were stained with FluoTag-X4 anti-GFP Nb (A–I), antibodies against GT335 and FOP (A′–I′), and against TCTN1 (A′′–I′′). The stained cells were then observed by Airyscan superresolution microscopy. Scale bars, 1 µm. (J–L) Relative TZ staining intensities of EGFP-fused MKS1 (J), B9D1 (K), and B9D2 (L) in the images acquired by confocal laser-scanning microscopy were estimated and expressed as scatter plots. In the scatter plots, different colored dots represent three independent experiments, horizontal lines are means, and error bars are SD. Statistical significances among multiple cell lines were calculated using one-way ANOVA followed by the Dunnett multiple comparison test.