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. 2020 Sep 29;11:566117. doi: 10.3389/fmicb.2020.566117

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Copyright © 2020 Wang, Chen, Liu, Sun and Zhang.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Scheme of the biosynthetic pathway of myo-inositol and the metabolic regulation strategy in Synechocystis. The genetic modifications made in this study were highlighted. The conversion of glucose-6-phosphate (G-6-P) to myo-inositol (MI) was catalyzed by inositol-1-phosphate synthase (INO1) and myo-inositol-1-monophosphatase (Sll1329 and Sll1383). The phosphoglucose isomerase (Pgi), phosphofructokinase (PfkA), and glucose-6-phosphate dehydrogenase (Zwf) were downregulated by a small RNA tool to direct G-6-P to MI biosynthesis.