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. 2020 Sep 29;10:563255. doi: 10.3389/fonc.2020.563255

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Copyright © 2020 Chen, Miao, Yang, Yin, Zhao and Song.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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NR_027471 inhibited the migration, invasion, and EMT of osteosarcoma cells. (A) The migration ability of osteosarcoma cells after overexpression or knockdown of lncRNA NR_027471 was determined using the scratch assay. (B) The invasion ability of osteosarcoma cells after overexpression or knockdown of lncRNA NR_027471 was determined using the Transwell Matrigel assay. (C) Quantitative analysis of the invasion ability of osteosarcoma cells after overexpression or knockdown of lncRNA NR_027471 was determined using the Transwell Matrigel assay. (D) The protein expression of E-cadherin, ZEB1, Snail, and fibronectin in osteosarcoma cell lines. Data are expressed as the mean ± SD of three independent experiments. ^**P < 0.01 compared with the pLVX-Vector group. ^##P < 0.01 compared with the pLKO.1-Vector group. EMT, epithelial–mesenchymal transition; lncRNA, long non-coding RNA; ZEB1, zinc finger E-box-binding homeobox 1; SD, standard deviation.