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. 2020 Sep 29;10:563255. doi: 10.3389/fonc.2020.563255

Figure 5.

Figure 5

LncRNA NR_027471 regulates the function of osteosarcoma cells by sponging miR-8055. (A) MS2b-based RIP assay with anti-GFP antibody (cross-reacting with YFP) in U2OS 48 h after transfection with MS2bp-YFP plasmid along with MS2bs-NR_027471, or MS2bs-Rluc (control vectors). (B) Binding of lncRNA NR_027471 and miR-8055 detected using the luciferase assay. (C) Binding of lncRNA NR_027471 and miR-8055 using the biotin-miRNA RIP assay. (D) Viability of U2OS cells transfected with mimic miR-8055 or inhibitor miR-8055 using CCK-8. (E) Viability of Saos-2 cells transfected with a mimic or inhibitor of miR-8055 using CCK-8. (F) Transwell invasion assay determined the invasion ability of U2OS and Saos-2 cells transfected with a mimic or inhibitor of miR-8055. (G) The Transwell invasion assay determined the invasion ability of U2OS and Saos-2 cells after co-transfection with the miR-8055 inhibitor and pLKO.1-NR_027471. (H,I) Histogram presenting the number of invasive cells. Data are expressed as the mean ± SD of three independent experiments. **P < 0.01. lncRNA, long non-coding RNA; RIP, RNA immunoprecipitation; YFP, yellow fluorescent protein; GFP, green fluorescent protein; CCK-8, Cell Counting Kit-8; SD, standard deviation.