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. 2020 Sep 29;11:561390. doi: 10.3389/fimmu.2020.561390

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Copyright © 2020 Sabbatino, Scognamiglio, Liguori, Marra, Anniciello, Polcaro, Dal Col, Caputo, Peluso, Botti, Zeppa, Ferrone and Pepe

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Representative staining patterns of formalin-fixed, paraffin-embedded thin melanoma lesions with CD8- (A), FOXP3- (A), GRZ-B- (B), HLA class I antigen- (C) and PD-L1- (E) specific mAbs. Double IHC staining was performed utilizing CD8- (brown cells) and FOXP3- (red cells) specific mAbs (A). For HLA class I antigen detection tissue sections were IHC stained with a pool of mouse HLA-A–specific mAb HCA2 and HLA-B/C-specific mAb HC10 (ratio, 1:1). mAbs E7Q5L (IgG2b) and DA1E (IgG) were used as isotype controls for HLA class I antigen (D) and PD-L1 (F) staining, respectively. Magnification is 200X.