Fig. 5.

Putative MKK4 homodimer and its dynamics. (A) Assembly of autoinhibited MKK4 homodimer. Phosphorylation patterns in simulated MKK4 dimer systems. Systems are coloured as: Up-DIM, blue; ppSA-DIM, green; ppSA/ppSB-DIM, pink; Up(R134W)-DIM, yellow. (B) Root-mean-square fluctuation (RMSF) of protein backbone. Average of five replicas is shown with standard deviation (thin vertical lines). Highlighted regions indicate activation segment (ILE250–SER292), green; C-lobe loop (PRO308– PHE340), dark orange. RMSF value of 0.4 nm is indicated with the horizontal dashed black line. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)