Fig. 3.

Signaling in cells expressing either A_2AR or A_2A^Δ40R. Dose response curves on 0.5 µM forskolin-induced cAMP levels, on ß-arrestin recruitment, on ERK1/2 phosphorylation, and on dynamic mass redistribution (DMR). Data (n = 12, each in triplicates) for cAMP are given in percentage (100% represents the forskolin effect). Data (n = 10, each in triplicates) for BRET assays, used to determine β-arrestin recruitment, are given in milliBRET Units (mBU). Data (n = 6, each in triplicates) for ERK1/2 phosphorylation are expressed as % with respect to basal levels. DMR tracings are representing the picometer (pm)-shifts of reflected light wavelengths over time upon ligand treatment.