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. 2020 Aug 14;44(5):1949–1960. doi: 10.3892/or.2020.7735

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Copyright: © Takei et al.

This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, reproduction and adaptation in any medium and for any purpose provided that it is properly attributed. For attribution, the original author(s), title, publication source (PeerJ) and either DOI or URL of the article must be cited.

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Figure 1. — Confirmation of defucosylation of 5-mG_2a-f by enzyme-linked immunosorbent assay (ELISA) using lectins. (A) C₄₄Mab-5 and 5-mG_2a-f were immobilized and incubated with biotin-labeled concanavalin A (Con A), followed by peroxidase-conjugated streptavidin. The enzymatic reaction was produced using a 1-Step Ultra TMB-ELISA. (B) C₄₄Mab-5 and 5-mG_2a-f were immobilized and incubated with biotin-labeled Aleuria aurantia lectin (AAL), followed by peroxidase-conjugated streptavidin. The enzymatic reaction was produced using a 1-Step Ultra TMB-ELISA. (C) C₄₄Mab-5 and 5-mG_2a-f were immobilized and incubated with biotin-labeled Pholiota squarrosa lectin (PhoSL), followed by peroxidase-conjugated streptavidin. The enzymatic reaction was produced using a 1-Step Ultra TMB-ELISA.