Figure 3.
Effect of Res on ROS production in H446 cells is detected by flow cytometry. The results were expressed as the mean ± standard deviation of at least 3 separate experiments. (A-D) Representative flow cytometric histograms of ROS content from the control (Ctl), Res, NAC+Res and NAC groups groups, respectively. (E) Results of ROS generation analysis. Res at 40 µg/ml increased the content of ROS compared with that of control (*P<0.05), while pretreatment of NAC at 2 mmol/l decreased the generation of ROS in cells stimulated by Res (#P<0.05 vs. the Res group). Res, resveratrol; ROS, reactive oxygen species; NAC, N-acetyl-L-cysteine.