Figure 3.

FBLN5 inhibits ovarian cancer cell proliferation in vitro and in vivo. The (A) growth curve and (B) colony formation assay were used to assess the effect of FBLN5 on the proliferation of ovarian cancer cells. (C) The HEY cell line with or without FBLN5 overexpression was injected subcutaneously into nude mice (n=5). (D) The tumor weights in each group were assessed. Data are presented as the mean ± standard error of the mean. n=5. (E) p21 and p27 expression was measured using western blot analysis in ovarian cancer cell lines with FBLN5 overexpression or knockdown. Data are presented as the mean ± standard error of the mean. n=3. *P<0.05, **P<0.01, ***P<0.001. FBLN5, fibulin-5; si, small interfering; NC, negative control.