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. 2020 Sep 3;44(5):2143–2151. doi: 10.3892/or.2020.7749

Figure 5.

Figure 5.

FBLN5 is the target gene of miR-27a-3p. (A) The mRNA level of miR-27a-3p was higher in HGSOC tissues compared with that in FT tissues, using RT-qPCR. (B) Correlation analysis of FBLN5 and miR-27a-3p expression levels. (C) Prediction of the binding sites between miR-27a-3p and 3′-UTR of FBLN5. The binding sequence of miR-27a-3p with the FBLN5 3′UTR sequence was deleted in the mutated type. Dual-luciferase reporter assays were used to analyzed the luciferase activity of the WT or MT FBLN5 3′UTR. (D) The mRNA expression level of miR-27a-3p was detected using RT-qPCR following SKOV3 cell transfection with miR-27a-3p mimics or inhibitor. RT-qPCR and western blot analysis revealed an inverse association between FBLN5 and miR-27a-3p expression. Upregulation of miR-27a-3p inhibited FBLN5 expression level and downregulated miR-27a-3p expression level could promote the expression level of FBLN5. Data are presented as the mean ± standard error of the mean. **P<0.01. FBLN5, fibulin-5; miR-27-3p, microRNA-27-3-p; HGSOC, high-grade serous ovarian cancer; FT, fallopian tube; RT-qPCR, reverse transcription-quantitative PCR; UTR, untranslated region; WT, wild-type; MT, mutant; NC, negative control.