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. 2020 Sep 14;44(5):1997–2008. doi: 10.3892/or.2020.7765

Figure 5.

Figure 5.

Curcumin affects the expression levels of Fas death receptor pathway-associated proteins/genes and tumor metastasis-associated proteins. (A) Following treatment with curcumin (20 µM) and 5-FU (500 µM), the expression levels of Fas death receptor signaling pathway-associated genes were assessed in HCT-116 cells using quantitative PCR analysis. (B) Following treatment with three different doses of curcumin (10, 20 and 30 µM) and 5-FU (500 µM) for 24 h, western blotting was used to determine the expression levels of various proteins (NF-κB, Fas, FADD, caspase-8, cleaved-caspase-8, caspase-3, cleaved-caspase-3, E-cadherin and claudin-3) in HCT-116 cells. (C) Semi-quantification of the relative protein expression levels. Results are presented as the mean ± standard error of the mean (n≥3). *P<0.05 and **P<0.01 vs. control group. 5-FU, 5-fluorouracil; CUR(L), 10 µM curcumin; CUR(M), 20 µM curcumin; CUR(H), 30 µM curcumin; FADD, Fas-associated protein with death domain; NF-κB, nuclear factor-κB.