Figure 3.

Inhibition of CK1α induces apoptosis in AML cells. (A) Three AML cell lines HL-60, THP-1 and HEL as well as patient blast cells were treated with 0, 20, 40 µM D4476 for 48 h, and the apoptosis was determined by Annexin V-FITC/PI. Meanwhile, the cleavage of PARP and the expression of anti-apoptotic protein survivin were also assessed. Images representing four independent experiment are shown. (B) HL-60, THP-1 and HEL cells were treated with lentivirus-mediated shRNA for 72 h, and the apoptosis was subsequently determined using Annexin V-APC/7AAD. The cleavage of PARP and the expression of survivin were also determined. Images representing four independent experiment are shown. CK1α, casein kinase 1α; AML, acute myeloid leukemia; PARP, poly(ADP-ribose) polymerase.