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. 2020 Sep 22;44(5):2021–2030. doi: 10.3892/or.2020.7776

Figure 3.

Figure 3.

SPAG6 expression inhibits the apoptosis of BL cells. (A and B) Annexin V-PI staining was used to detect the apoptosis rate of control groups (nc-shRNA) and SPAG6 knockdown (shSPAG6) groups by flow cytometry assay in Daudi and Raji cells. (C) The levels of apoptosis-related proteins (c-MYC, Bcl-2, Bax, caspase-8, cleaved-caspase-8, caspase-3, cleaved-caspase-3, PARP and cleaved-PARP) were examined by western blot analysis after knockdown of SPAG6 in Daudi and Raji cells. *P<0.05, **P<0.01. The relative expression of apoptosis-related proteins of shSPAG6 group was compared with the nc-shRNA group. SPAG6, sperm-associated antigen 6; BL, Burkitt lymphoma; PARP, poly(ADP-ribose) polymerase.