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. 2020 Sep 22;44(5):2021–2030. doi: 10.3892/or.2020.7776

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Copyright: © Zhang et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 4. — SPAG6 promotes proliferation of BL cells through the PTEN/PI3K/AKT pathway. (A) The levels of PI3K/AKT pathway-related proteins (AKT, p-AKT and PTEN) were examined in SPAG6-depleted (shSPAG6) or control (nc-shRNA) Daudi and Raji cells by western blot analysis. (B) Stable SPAG6-knockdown Daudi and Raji cells were transfected with PTEN siRNA (siPTEN) and the knockdown efficiency was confirmed by western blot assay. (C) Cell viability in the nc-shRNA, shSPAG6 and shSPAG6/siPTEN groups was examined using the CCK-8 assay. (D) Cells were treated with DMSO or SF1670 (10 µM) for 24 h and cell viability in the nc-shRNA/DMSO, shSPAG6/DMSO and shSPAG6/SF1670 groups was examined using the CCK-8 assay. (E) The levels of PI3K/AKT pathway-related proteins (AKT, p-AKT and PTEN) were examined in SPAG6-overexpressing (pcDNA-SPAG6) or control (pcDNA) CA46 and NAMALWA cells by western blot analysis. (F) Stable SPAG6-overexpression CA46 and NAMALWA cells were transfected with PTEN overexpression plasmid (PTEN) and the overexpression efficiency was confirmed by western blot anallysis. (G) Cell viability in the pcDNA, pcDNA-SPAG6 and pcDNA-SPAG6/PTEN groups was examined using the CCK-8 assay. (H) Cells were treated with DMSO or LY294002 (20 µM) for 24 h and cell viability in the pcDNA/DMSO, pcDNA-SPAG6/DMSO and pcDNA-SPAG6/LY294002 groups was examined using the CCK-8 assay. *P<0.05, **P<0.01; ***P<0.001; ns, not significant. SPAG6, sperm-associated antigen 6; BL, Burkitt lymphoma; PTEN, phosphatase and tensin homolog; PI3K, phosphoinositide 3-kinase; CCK-8, Cell Counting Kit-8.