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. 2020 Sep 7;44(5):2009–2020. doi: 10.3892/or.2020.7758

Figure 4.

Figure 4.

circNOX4 promotes the progression of CRC by sponging miR-485-5p. (A and B) The enrichment of miR-485-5p was detected in (A) SW480 and (B) SW620 CRC cells transfected with si-NC, si-circNOX4, si-circNOX4 + anti-miR-NC or si-circNOX4 + anti-miR-485-5p by reverse transcription-quantitative PCR. (C and D) The proliferation of (C) SW480 and (D) SW620 CRC cells was determined by MTT assay. (E and F) The (E) migration and (F) invasion of CRC cells were detected by Transwell assays. (G and H) The glucose consumption and lactate production in transfected SW480 and SW620 cells were measured. (I-L) The (I and J) ECAR and (K and L) OCR of SW480 and SW620 cells transfected with si-NC, si-circNOX4, si-circNOX4 + anti-miR-NC or si-circNOX4 + anti-miR-485-5p were detected. (M and N) The protein expression levels of GLUT1 and LDHA in (M) SW480 and (N) SW620 CRC cells were detected by western blot assay. *P<0.05. circNOX4, circular RNA NADPH oxidase 4; miR, microRNA; CRC, colorectal cancer; si, small interfering RNA; NC, negative control; ECAR, extracellular acidification rate; OCR, O2 consumption rate; OM, oligomycin; FCCP, p-trifluoromethoxy carbonyl cyanide phenylhydrazone; Rote/AA, antimycin A plus rotenone; 2-DH, 2-deoxyglucose; GLUT1, glucose transporter 1; LDHA, lactate dehydrogenase A.