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Effect of compounds 1, 2, and 4–6 on LPS-induced protein expressions of iNOS and COX-2 in RAW 264.7 cells. The cells were treated with 20 μM of compounds for 30 min prior to LPS (1 μg/mL) treatment and incubated for 18 h. Protein expression levels were determined by Western blotting analysis. Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) was used as the internal standard.
