Figure 5.

(A–G) Systemic inflammation markers evaluated in plasma of CD and AGE-D mice by multiplexed Bio-Plex 3D system, indicating increased pro-inflammatory (IL-1β, IL-17, IFN-γ, TNF-α, and PAI-1) and decreased IL-6 and IL-10. (H) Activity of intestinal alkaline phosphatase (IAP) evaluated in plasma by kinetic assay, indicating reduced ability of AGE-D mice to maintain microbiota homeostasis and loss of detoxifying potential in intestine. Data are means ± S.E.M. (n = 15 per group). Statistical significance: * p < 0.05, ** p < 0.01 vs. CD.