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. 2020 Sep 22;44(5):2198–2210. doi: 10.3892/or.2020.7778

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Copyright: © Jotatsu et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 3. — Expression, secretion, and uptake of CD81-Nluc. (A) MeT-5A cells expressing CD9-Nluc, CD63-Nluc, and CD81-Nluc were cultured with medium containing FBSpas50 for 24 and 48 h. Nluc activity in each EVpas220 medium and cell lysate was measured. Nluc activity of each cell lysate was set to 1. The experiment was performed three times independently; bars, SD. **P<0.01 vs. each 24 h. (B) MeT-5A cells expressing CD81-Nluc were cultured in medium containing FBSpas50 for 48 h. Nluc activities of EVpas220, EVpas50/220 and EVpas20/50 were measured. Nluc activity of EVpas220 was set to 1. The experiment was performed three times independently; bars, SD. **P<0.01. (C) MeT-5A cells expressing CD81-Nluc were cultured in medium containing FBSpas50 for 48 h. MeT-5A and IHPF cells were cultured with EVpas220 and EVpas50/220 for the indicated hours and each Nluc of cell lysate was measured. The value of Nluc activity with EVpas50/220 was subtracted from its value with EVpas220. Nluc activity of MeT-5A cells cultured for 6 h was set to 1.