Figure 3.

miR-216a-5p suppresses the proliferation of ESCC cells in vitro. (A) The proliferation ability of EC9706 and TE1 cells transfected with the miR-216a-5p mimic, or the miR-216a-5p inhibitor, or the corresponding controls (mimic NC or inhibitor NC) were determined by CCK-8 assays. *P<0.05, compared with the mimic NC or inhibitor NC. (B) The proliferation abilities of EC9706 and TE1 cells after the indicated transfections were evaluated by colony formation assays. The representative images of cells in a 6-well plate after crystal violet staining are shown. *P<0.05, **P<0.01 and ***P<0.001. (C) The cell cycle progression of EC9706 and TE1 cells after the indicated transfections were determined by flow cytometry. The representative flow profiles of DNA content after PI staining are shown. ESCC, esophageal squamous cell carcinoma.