Figure 5.

Ectopic KIAA0101 expression attenuates the anti-proliferative effects of miR-216a-5p in ESCC cells. (A) Western blot analysis was used to determine the expression level of KIAA0101 protein after mock transfection, or transfection of miR-216a-5p mimic alone (miR-216-5p), or combined transfection of miR-216a-5p mimic and KIAA0101-expressing plasmid (miR-216-5p+KIAA0101). Representative western blot images are shown, and the relative expression level of KIAA0101 from three independent experiments was summarized. **P<0.01 and ***P<0.001. (B) The proliferation of EC9706 cells after the indicated transfections was determined by CCK-8 assay. n=3 for each group at each time point. *P<0.05, compared with the Mock group. (C) The proliferation of EC9706 cells after the indicated transfections was determined by colony formation assays. The representative images of crystal violet staining are shown, and the numbers of colonies per well were summarized. n=3 for each group. **P<0.01 and ***P<0.001. (D) The cell cycle progression of EC9706 and TE1 cells after the indicated transfections was determined by flow cytometry. The representative flow profiles of DNA content after PI staining are shown. ESCC, esophageal squamous cell carcinoma.