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. 2020 Sep 22;12(9):1054. doi: 10.3390/v12091054

Figure 4.

Figure 4

Western blot analysis of WT MHV-E protein expression and mutants following expression in insect cells. (A) Western blot using anti-His antibody. Lane M - See Blue™ Plus2 Pre-Stained Protein Standard (Invitrogen). Lanes 1-11 are samples as follows: 1: WT E, 2: L50A, 3: V51A, 4: L52A, 5: P54A, 6: Y57A, 7: Y59A, 8: all mutants, 9: deleted EPTM, 10: GFP baculovirus (positive control), 11: mock. (B) Western blot by anti baculovirus surface glycoprotein gp64 following stripping of the membrane used for panel A Key molecular mass markers are indicated to the left of each panel.