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. 2020 Aug 19;44(5):2067–2079. doi: 10.3892/or.2020.7743

Figure 5.

Figure 5.

eIF3a is a downstream target of miR-875-5p in HCC cells. (A) Results from TargetScan (http://www.targetscan.org) and miRanda (microRNA.org) showed the predicted miR-875-5p binding sites in 3′UTR of eIF3a mRNA. (B) Luciferase reporter assay indicated that alteration of miR-875-5p expression inversely regulated luciferase activities of wild-type (wt) but not mutant (mt) eIF3a 3′UTR plasmids. (C and D) RT-qPCR and western blotting were performed to identify the expression of eIF3a mRNA and protein in Hep3B cells transfected with miR-875-5p inhibitors. (E and F) HCCLM3 cells with miR-875-5p knockdown. (G) RT-qPCR was performed to detect eIF3a mRNA expression in 90 pairs of HCC tumor tissues and corresponding adjacent non-tumor tissues. (H) Pearson's correlation analysis disclosed the negatively correlation between eIF3a mRNA and miR-875-5p in HCC tissues. (I) Western blotting was performed to detect eIF3a expression of HCC tissues with low miR-875-5p expression and high miR-875-5p expression. (J) Western blotting was performed to determine eIF3a expression in mice xenograft tumor tissues. *P<0.05.