Table 4.
Fractions | The diameter of zone of inhibition (mm) |
||||||||
---|---|---|---|---|---|---|---|---|---|
Gram-positive |
Gram-negative |
Fungi |
|||||||
SA | MRSA | PA | EC | KP | SS | ST | CA | CN | |
PE | 7.7 ± 0.3ab | NT | 0b | 8.3 ± 0.3ab | 0b | 0b | 0b | 7.7 ± 0.3ab | NT |
DCM | 7.7 ± 0.3ab | 0b | 0b | 8.0±0ab | 0b | 7.7 ± 0.3ab | 0b | 7.3 ± 0.3ab | 0b |
EA | 8.7 ± 0.3ab | 0b | 0b | 0b | 0b | 0b | 0b | 0b | 6.7 ± 0.3ab |
MeOH | 8.3 ± 0.3ab | 6.3 ± 0.3ab | 0b | 8.0±0ab | 7.0±0ab | 8.0±0ab | 6.7 ± 0.3ab | 8.3 ± 0.3ab | NT |
PC | 33.7 ± 0.3 | 24.3 ± 0.3 | 23.7 ± 0.6 | 15 ± 0 | 15.7 ± 0.3 | 19.7 ± 0.6 | 21.3 ± 0.3 | 16.3 ± 0.3 | 20.3 ± 0.3 |
NC | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
PE: Petroleum ether fraction at 5 μg/disc; DCM: Dichloromethane fraction at 5 μg/disc; EA: Ethyl acetate fraction at 2.5 μg/disc; MeOH: Methanol fraction at 5 μg/disc; PA: Pseudomonas aeruginosa; EC: Escherichia coli; SA: Staphylococcus aureus; KP: Klebsiella pneumoniae; MRSA: Methicillin Resistant Staphylococcus aureus; SS: Shigella sonnei; ST: Salmonella typhi; CA: Candida albicans; CN: Cryptococcus neoformans; PC: Positive control (Oxacillin 10 μg/disc and Gentamycin 10 μg/disc for Gram positive and Gram negative bacteria respectively. Nystatin 100 μg/disc for fungi); NC: Negative control (Discs loaded with 10 μl of 1% DMSO); n = 3; values = Mean ± SEM; Values followed by similar superscript letters are not significantly different from each other (ANOVA - Tukey's post-hoc multiple comparisons, P < 0.05).