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. 2020 Aug 20;12(9):2514. doi: 10.3390/nu12092514

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Broccoli feeding increases protein expression of Nqo1 and Nrf2 in rats after 14 days of broccoli feeding (A) Timeline of study. Rats were fed a diet with or without 10% freeze-dried broccoli (n = 4/feeding group). (B) The protein expression of Nqo1 and Nrf2 in liver tissue was detected with Western blot. β-actin served as a loading control, used to normalize Nqo1 and Nrf2 protein expression and calculate data for C,D. (C) Quantification of Western blot results. Unpaired t-test analysis of Nqo1(C) and Nrf2 (D) protein expression. p < 0.05 as compared with the control group, all data and standard deviation are plotted.