Table 5.
1D-LC Separation | 2D-LC Separation | Valve Position | ||||
---|---|---|---|---|---|---|
Time (min) | A (%) | B1 (%) | Time (min) | A (%) | B2 (%) | |
0 | 50 | 50 | 0 | 45 | 55 | 6-1 |
0.5 | 50 | 50 | 0.5 | 45 | 55 | 1-2 |
1.9 | 50 | 50 | 1.9 | 45 | 55 | |
3.0 | 50 | 50 | 2.7 | 45 | 55 | 6-1 |
8.0 | 2 | 98 | 3.0 | 90 | 10 | |
11 | 2 | 98 | 5.0 | 90 | 10 | |
11.5 | 90 | 10 | 9.0 | 2 | 98 | |
13 | 90 | 10 | 11 | 2 | 98 | |
13.5 | 60 | 40 | 11.2 | 45 | 55 | |
16 | 60 | 40 | 16 | 45 | 55 |
A: deionised water containing 0.1% (v/v) of formic acid, B1: 0.5 mM ammonium acetate in acetonitrile containing 0.1% (v/v) of formic acid, B2: acetonitrile containing 0.1% (v/v) of formic acid.