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. 2020 Aug 29;12(9):555. doi: 10.3390/toxins12090555

Table 5.

Gradient eluting programme and six-port valve-switching information.

1D-LC Separation 2D-LC Separation Valve Position
Time (min) A (%) B1 (%) Time (min) A (%) B2 (%)
0 50 50 0 45 55 6-1
0.5 50 50 0.5 45 55 1-2
1.9 50 50 1.9 45 55
3.0 50 50 2.7 45 55 6-1
8.0 2 98 3.0 90 10
11 2 98 5.0 90 10
11.5 90 10 9.0 2 98
13 90 10 11 2 98
13.5 60 40 11.2 45 55
16 60 40 16 45 55

A: deionised water containing 0.1% (v/v) of formic acid, B1: 0.5 mM ammonium acetate in acetonitrile containing 0.1% (v/v) of formic acid, B2: acetonitrile containing 0.1% (v/v) of formic acid.