Table 1.
The list of antibodies used for immunohistochemistry.
| Antibody (Clone Name) |
Type | Dilution | Pretreatment | Source | Code |
|---|---|---|---|---|---|
| CD3 (F7.2.38) |
Mouse monoclonal |
1:500 | Microwaving in 10 mM Tris-EDTA (pH 9.0) for 20 min | Dako | M7254 |
| CD68 (ED-1) |
Mouse monoclonal |
1:500 | ProK (100 μg/mL) for 10 min | Millipore | MAB1435 |
| γH2A.X (20E3) |
Rabbit monoclonal |
1:500 | Microwaving in 10 mM citrate buffer (pH 6.0) for 20 min | Cell Signaling Technology | #9718 |
EDTA; ethylenediaminetetraacetic acid, ProK; proteinase K, γH2A.X; phosphorylated histone H2A.X.